FIGURE 2.

Splenic IM are localized largely within the MZ in naive mice and depleted following DT injection. Confocal microscopy of spleen sections from nonchimeric CD11b-DTR mice with or without injection of DT 26 h earlier and with injection of Alexa Fluor 405-R614 (red) 2 h earlier. Sections subsequently stained with PE–anti-Ly6C (blue) or eGFP (green). One of two representative experiments. Original magnification ×20.