FIGURE 7.

IM are phenotypically and functionally distinct from circulating CD11b⁺CD11c^lowLy6G⁻/C⁻ cells (immature blood DC). (A) Expression of CD11c (PE–anti-CD11c) on gated CD19⁻CD90⁻Ly6G⁻ cells (Alexa Fluor 700–anti-CD19, anti-CD90, and anti-Ly6G) from naive WT mice followed by Ly6C (FITC–anti-Ly6C) and CD11b (BV421–anti-CD11b) expression on gated CD11c^neg, CD11c^low, and CD11c^high cells. (B) WT mice were injected with Alexa Fluor 405–labeled R614 (red). Four hours postinjection, spleens were obtained, and sections were stained with FITC–anti-Ly6C (green) plus PE–anti-CD11b (blue). Original magnification ×63 oil.