. Author manuscript; available in PMC: 2013 Jan 19.

Published in final edited form as: Radiat Res. 2011 Oct 26;177(1):69–83. doi: 10.1667/rr2524.1

TABLE 3.

Validation of Microarray Results by RT-qPCR

Gene	Postirradiation time and dose	qPCR mRNA ratio (radiation/control)		Microarray mRNA ratio (radiation/control)
Gene	Postirradiation time and dose	B2M as reference gene	GAPDH as reference gene	Microarray mRNA ratio (radiation/control)
CDKN1A	8 h, 5 Gy	3.2 (P1)	4.0 (P1)	1.8 (P1+P2)
	30 h, 5 Gy	3.2 (P1)	4.0 (P1)	2.9 (P1+P2)
CLDN1	30 h, 0.05 Gy	2.5 (P1+P2)	1.5 (P1+P2)	1.6 (P1+P2)
EGFR	30 h, 0.05 Gy	20.2 (P1+P2)	3.9 (P1+P2)	2.1 (P1+P2)
ING2	30 h, 0.05 Gy	1.2 (P1+P2)	3.1 (P1+P2)	1.8 (P1+P2)
	30 h, 5 Gy	1.6 (P1)	1 (P1)	2.0 (P1+P2)
SESN1	8 h, 5 Gy	1.5 (P1)	1.9 (P1)	1.9 (P1+P2)
	30 h, 5 Gy	4.0 (P1)	2.5 (P1)	1.9 (P1+P2)

Notes. RT-qPCR was performed on total RNA extracted from additional skin samples from each patient (P1 and P2); additional skin samples for 8 h and 30 h after exposure to 5 Gy were not available for patient P2. Expression of five selected genes and two reference genes was analyzed. Calculated gene expression changes based on RT-qPCR analysis represent either a mean between the two patients, or an expression change in one patient (P1). Calculated gene expression changes based on microarray analysis were calculated from the ANOVA coefficients.