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General scheme for affinity protection chromatography. To ensure the label on an antibody or affinity ligand does not interfere with analyte binding, an analog of the analyte is immobilized onto a solid support. The antibody is then applied and allowed to bind the analyte. The reaction to label the antibody is then performed. Once the reaction is complete, the antibody can be eluted from the solid support. This figure is adapted from Ref. [115].
