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3D Stack images of multiphoton-induced fluorescence of HEK293 expressing EGFP-Rheb. Fluorescence from TCSPC images of live HEK293 cells was acquired using multiphoton excitation (920 nm laser excitation, 520 nm emission) following 24h transfection. Raw data presented without further image processing. Images show clear Rheb nuclear localization. Yellow and red boxes (F) refer to nuclear and ER/Golgi regions taken for comparative photon count. Image size 120x100 μm.
