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Curry leaf extract inhibits activity of purified 20S proteasome. Purified 20S proteasome from rabbit was incubated with 40 μM of fluorogenic peptide substrate for Ch-L activity in the presence of various concentrations of the CLE for 2 h at 37°C. The fluorescence intensity of the free AMCs was determined using fluorescence mode in a multimode reader with excitation (380 nm) and emission (460 nm).
