Skip to main content
. Author manuscript; available in PMC: 2014 Jan 18.
Published in final edited form as: Circ Res. 2012 Nov 14;112(2):298–308. doi: 10.1161/CIRCRESAHA.112.274803

Figure 1. RyR2-V2475F channels display higher sensitivity to cytosolic Ca2+ than WT channels.

Figure 1

A, Ca2+-dependence of [3H]ryanodine binding curves performed in parallel using the solubilized and purified channels indicated in the inset. Asterisk indicates p<0.05. B, Single channel recordings of WT and V2475F channels reconstituted in lipid bilayers. Left panels show 2-sec segments of representative single channel activity at the indicated cis (cytosolic) Ca2+ and the right panels are current histograms of at least 5 min of cumulative recording performed in n=6 (WT) and n=4 (V2475F) channels