Figure 1.

Oil Red O (ORO) staining in cells (SUM149, MDA-MB-231, and MCF10a) with FBS control, and treated with LPDS and LDL. (a) Representative bright field images for each treatment. The scale bars represent 10 μm. (b) Quantitation of relative stain intensity (total stain area/number of cells) was performed for each image using ImageJ. Statistical significance (*P < 0.01) was determined by one-way ANOVA followed by Tukey's HSD test. The error bars represent SEM. All experiments were performed at least three separate times.