Figure 5.
Periplocin treatments affected tumor growth in xenograft in vivo model. HCC cells were subcutaneously injected into SCID mice at 3 × 106 cells. Periplocin was IP injected into SCID mice daily two weeks after initial tumor injection (5 mg/kg on day 15 to day 29 and 20 mg/kg on day 29 to day 35). (a) Tumor size was measured every 2–4 days throughout the study (*P < 0.05). (b) Body weight of control and treated mice were measured every 2–4 days throughout the study. (c) After the mice were sacrificed, tissue sections were prepared and stained with anti-Ki67 and anticyclin-D1 antibodies to evaluate cell proliferation. (d) Huh-7 cells were fixed, and immunofluorescent stained by Hoechst 33258 (blue staining), Ki67 (red staining), and cyclin-D1 (green staining).