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. Author manuscript; available in PMC: 2013 Jan 20.
Published in final edited form as: Dev Cell. 2008 Sep;15(3):386–400. doi: 10.1016/j.devcel.2008.07.005

Figure 3. Cdc42 activation requires spindle pole attachment to the oocyte cortex.

Figure 3

A. A series from 4D movie of an oocyte injected with C3 mRNA 60 min following GVBD to inhibit spindle rotation (Alexa 488 anti-Aur B, green; rhodamine-tubulin, red). Note the complete separation of chromosome homologs with spindle midzone clearly visible (arrows, 00:30), and the subsequent re-congressing of all chromosomes.

B. A series from 4D movie showing an oocyte injected with C3 mRNA 60 min after GVBD (eGFP-wGBD, green; rhodamine-tubulin, red). Note the complete absence of Cdc42 activation.

C. A series from 4D movie of an oocyte injected with C3 mRNA 90 min following GVBD (eGFP-wGBD, green; rhodamine-tubulin, red). Note the limited Cdc42 activation (arrow) that did not develop into a robust cap. No polar body formed.