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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Rep Prog Phys. 2012 Dec 18;76(1):016601. doi: 10.1088/0034-4885/76/1/016601

Figure 5.

Figure 5

Model and experimental observation of tRNA accommodation in the ribosome. (a) A revised model of two-staged tRNA selection (initial selection and proofreading step that follows GTP hydrolysis) based on smFRET experiments (Blanchard et al., 2004). (b) Time evolution of FRET after the initial binding of cognate correct tRNA when GTP hydrolysis is allowed so that both stages of tRNA selection can occur (Blanchard et al., 2004). (c) Time evolution of FRET comparing the delivery of correct (left) and nearly correct (right) tRNAs stalled by non-hydrolizable GTP analog, GDPNP, so that only the initial selection can occur. Both the mid-FRET and low-FRET efficiencies are higher for the correct tRNA by significant amount, suggesting that the tRNA selection has high fidelity even before GTP hydrolysis (Lee et al., 2007c).