. Author manuscript; available in PMC: 2013 Jan 20.

Published in final edited form as: Biochemistry. 2011 Nov 8;50(47):10195–10202. doi: 10.1021/bi2015019

Table 2.

Values of k_cat/K_m (µM⁻¹s⁻¹) for thrombin wild-type and mutants toward synthetic and physiological substrates

Enzyme	FPR	FpA	PAR1	Protein C^a
Wt	37±1	17±1	27±1	0.22±0.01
EDE	19±1	7.2±0.5	9.1±0.6	0.11±0.01
WE	0.0014±0.0001	0.00027±0.00001	0.0099±0.0003	0.027±0.001
EDEWE	0.0015±0.0001	0.00026±0.00001	0.016±0.001	0.031±0.001

EDE: E14eA/D14lA/E18A; EDEWE: E14eA/D14lA/E18A/W215A/E217A; FpA: fibrinopeptide A; FPR: H-D-Phe-Pro-Arg-p-nitroanilide; WE: W215A/E217A; Experimental conditions are: 5 mM Tris, 0.1% PEG 8K, pH 7.4, 37 °C, 145 mM NaCl.

In the presence of 100 nM thrombomodulin and 5 mM CaCl₂.