Quantitative analysis of BDNF, TrkB, phospho-TrkB protein, and TrkB mRNA in cortical neurons (DIV8) after BDNF treatment. Axons and dendrites are shown in gray and dark gray bars, respectively. Y-axis: I, intensity; A, Area; N, number. Student’s t test: *P < 0.05, **P < 0.01. See text for actual P-values. Error bars show standard deviations. A, B: Levels of BDNF expression in cortical neurons were detected before and after treatment with 2 nM BDNF for 1 h. Analysis performed on 0—30 μm and 30—60 μm segments is shown in (A) and (B), respectively; n = 5. C, D: Levels of TrkB in cortical neurons were detected before and after treatment with 2 nM BDNF for 1 h. Analysis performed on 0—30 μm and 30—60 μm segments is shown in (C) and (D), respectively; n = 5. E, F: Levels of phospho-TrkB in cortical neurons detected before and after treatment with 2 nM BDNF for 1 h. Analysis performed on 0—30 μm and 30—60 μm segments is shown in (E) and (F), respectively; n = 5. G, H: Levels of TrkB mRNA in cortical neurons detected before and after treatment with 2 nM BDNF for 1 h. Number-based analysis performed on 0—30 μm and 30—60 μm segments is shown in (G) and (H), respectively; n = 6.