Figure 5.

Hes4 overexpression delays cell cycle exit. (A, B): BrdU incorporation assay (3-hour pulse) at stage 37, following Hes4-GR mRNA injection. (A): Typical retinal sections immunostained with anti-BrdU. (B): Corresponding quantification. (C, D): Birthdating experiments in Hes4 lipofected retinas. (C): Typical retinal section immunostained for both GFP and BrdU, following continuous BrdU exposure from stage 34 to 41. (D): Percentage of BrdU⁺ cells among transfected cells. Scale bar = 50 μm. Abbreviations: BrdU, 5-bromo-2′-deoxyuridine; CMZ, ciliary marginal zone; CR, central retina; GR, glucocorticoid receptor; GFP, green fluorescent protein; L, lens.