FIGURE 1.

Myeloperoxidase (MPO) is required for PMA but not bacterial induction of NETs. (A,B) The release of NETs from control (filled bars) and MPO-deficient (open bars) neutrophils measured over 4 h. MPO-deficient neutrophils formed NETs less efficiently with PMA, but not with P. aeruginosa, than neutrophils from control donors. To inhibit MPO, samples were incubated in the presence of 100 μM of the MPO inhibitor 4-aminobenzoic acid hydrazide (ABAH). Results are means ± SEM of two to three independent experiments. For PMA, p = 0.02 at 180 min; p = 0.071 at 240 min by t-test. Data obtained with permission from Parker et al. (2012b). (C,D) Schematic representations of the intra- and extracellular locations of oxidant production in response to (C) soluble and non-phagocytic stimuli, or (D) phagocytosis (reviewed in Bylund et al., 2010 and Robinson, 2008). Details are given in the text. With PMA, oxidant production is predominately extracellular while phagocytosis induces largely intracellular production.