Figure 2.

Treatment of BMDC with E2 during T. muris E/S pulsing enhances their Th2 priming capacity. (A) BMDC were pulsed with T. muris E/S(C, thick black line) and in combination with 10⁻¹⁰ M E2 (grey fill) or with E2 and 10⁻⁸ M ICI 182 780 (ICI, dotted black line). The percent and MFI of CD11c⁺ DC expressing CD80 and CD86 and MHC class II are indicated. Isotype controls are indicated by thin black lines. (B) DC were co-cultured with purified CD4⁺ T cells and following stimulation cytokine production in the co-culture supernatants was assayed via CBA. All treatments represent triplicate samples±SEM and the data are representative of three independent experiments. ^*p≤0.05, ^**p≤0.01, ^***p≤0.001, Mann–Whitney t-test.