Figure 2.
TFPIγ mRNA is produced in mouse but not human tissues. DNA gel electrophoresis of PCR products from reactions designed to amplify TFPIα (5’ primer in second Kunitz domain; 3’ primer in C-terminal region of TFPIα) or TFPIβ and TFPIγ (5’ primer in the second Kunitz domain; 3’ primer in distal end of exon 8). Lane 1 – human TFPIβ (TFPIγ is not observed); Lane 2 – human TFPIα; Lane 3 – mouse TFPIβ and TFPIγ; Lane 4 – mouse TFPIα. Identical results were obtained using cDNA from human kidney and placenta.