Human 3' RACE |
|
Exon 6 outer |
5'-TGG GCA ATA TGA ACA ATT TTG AGA CAC-3' |
Exon 6 inner |
5'-GGA ATA TGT CGA GGT TAT ATT ACC AGG T-3' |
Exon 9 outer |
5'-GTG GAT GTG GGG GAA ATG AAA-3' |
Exon 9 inner |
5'-CTT CCA AAC AAG AAT GTC TGA GGG CAT-3' |
Mouse 3' RACE |
|
Exon 6 outer |
5'-GAA AGG CCA GAT TTC TGC TTC TTG GAA GAG-3' |
Exon 7 inner |
5'-CCC AGT CTC CCA AAG TGC CCA GGC GTC GGG-3' |
Primers used in
Figures 2 and 3C
|
|
Human K2 forward |
5'-GGA ATA TGT CGA GGT TAT ATT ACC AGG T-3' |
Human K3 reverse |
5'-GGC GGC ATT TCC CAA TGA CTG AAT-3' |
Human Exon 8 reverse |
5'-TGC ATG TAA ATA TTA AAA CTT TAT TAG-3' |
Mouse K2 forward |
5'-CCT GGG CAA CCG CAA CAA CTT-3' |
Mouse K3 reverse |
5'-CAT GAT CTC AGA CAT CTC CTT CTG-3' |
Mouse Exon 8 reverse |
5'-TGG CCA CAG GGT CTT CTT TAT TAC ATC T-3' |
Real time PCR |
|
Human TFPIα forward |
5'-ATT TCA CGG TCC CTC ATG GTG TCT-3' |
Human TFPIα reverse |
5'-GGC GGC ATT TCC CAA TGA CTG AAT-3' |
Human TFPIβ forward |
5'-GAA GGA ACA AAT GAT GGT TGG AAG AAT GCG-3' |
Human TFPIβ reverse |
5'-ATG GAT GCA TGA ATG CAG AAG GCG-3' |
Mouse TFPIα forward |
5'-CAA TTC AGC CAC TGG GAA ATG C-3' |
Mouse TFPIα reverse |
5'-CAT GAT CTC AGA CAT CTC CTT CTG-3' |
Mouse TFPIβ forward |
5'-CCC AGT CTC CCA AAG TGC CCA GGC GTC GGG-3' |
Mouse TFPIβ reverse |
5'-GAC GGA ACT CAG AAA GCC TTG GTA-3' |