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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Biochem J. 2012 Apr 1;443(1):259–266. doi: 10.1042/BJ20111994

Figure 5. Disruption of caveolae decreases the TF-fVIIa inhibitory activity of TFPI-GPI, but not TFPI-TM.

Figure 5

A) Time course of fXa (nM) generation by cholesterol-depleted CHO-TF cells (▲). Treatment with α-cyclodextrin (■) served as a negative control. B) Amount of fXa (nM) generated by cholesterol-depleted CHO-TF/TFPI-GPI and CHO-TF/TFPI-TM cells at different time points. (▼) methyl-β-cyclodextrin and (■) α-cyclodextrin -treated CHO-TF/TFPI-GPI cells; (◆) methyl-β-cyclodextrin and (▲) α-cyclodextrin -treated CHO-TF/TFPI-TM cells. For both figures, the total amount of fXa generated at the end of 200 second intervals of the progress curve are shown (a representative progress curve is presented in Figure 4A). Equivalent cell concentrations of approximately 300 µg/mL total cellular protein were used for all experiments; mean ± SD, n=4. The assay was performed with cells in suspension. # - p < 0.05 for α-CD- vs. m-β-CD-treated CHO-TF/TFPI-GPI cells. *** - p < 0.001 for α-CD- vs m-β-CD-treated CHO-TF/TFPI-GPI cells.