. Author manuscript; available in PMC: 2013 Apr 1.

Published in final edited form as: Biochem J. 2012 Apr 1;443(1):259–266. doi: 10.1042/BJ20111994

Table 2. Disruption of caveolae does not affect the fXa inhibitory activity of endogenous TFPI.

Cells in suspension were treated with α-cyclodextrin (α-CD) or methyl-β-cyclodextrin (m-β-CD) and the fXa inhibition assay performed at the cellular equivalent of 100 µg/mL total protein. p > 0.05 between α-CD and m-β-CD treated cells of the same cell line, n = 6 for each cell line.

		Free Factor Xa (nM)
Cell Line	Cyclodextrin Tx	Initial	Final
HUVEC	α-CD	0.098 ± 0.044	0.040 ± 0.017

	m-β-CD	0.090 ± 0.025	0.046 ± 0.012

HB2	α-CD	0.077 ± 0.016	0.067 ± 0.031

	m-β-CD	0.090 ± 0.038	0.061 ± 0.035

EA.hy926	α-CD	0.082 ± 0.038	0.060 ± 0.010

	m-β-CD	0.079 ± 0.017	0.065 ± 0.016