FIG. 4.

Tube formation by HUVECs in the presence of CD133⁺ cell-derived CM and MVs. (A) HUVECs were seeded on Matrigel in the presence of medium alone, CD133⁺ cell-derived MVs (30 μg/mL), CM from CD133⁺ cells, CM from CD133⁺ cells w/o MVs, or FGF-2 (50 ng/mL, positive control) in a serum-free medium and incubated for 18 h at 37°C. The experiment was independently repeated 3 times in quadruplicate, and the data were pooled together. *P<0.0001. (B) Representative image of tube formation by HUVECs in the presence of medium alone (negative control), CD133⁺ cell-derived MVs (30 μg/mL), platelet derived microvesicles (30 μg/mL), and FGF-2 (50 ng/mL).