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. 2012 Aug 8;22(3):492–500. doi: 10.1089/scd.2012.0308

FIG. 2.

FIG. 2.

Nmp4-KO BM yielded more osteogenic stem and progenitor cells irrespective of treatment. (A) Total number of CFU-FAlkPhos+ colonies in BM cultures derived from WT and Nmp4-KO mice treated with intermittent hPTH (1–34) 30 μg/kg/day or vehicle for 3 weeks. (B) Total number of CFU-F colonies. (C) The percent CFU-FAlkPhos+ colonies/total CFU-F colonies (average±SD, number of mice/experimental group=6–8; statistical differences determined by a 2-way ANOVA). (D) The frequency of femoral CD45−/CD146+/CD105+/nestin+ multipotent mesenchymal stem cells in untreated WT and Nmp4-KO mice; FACS was used to evaluate the BM from each mouse as described in the Materials and Methods section (average±SD, number of mice/experimental group=12–20; statistical difference was determined using a 2-sample t-test assuming unequal variances). BM, bone marrow; FACS, fluorescence activated cell sorting.