Table 2.
Immunophenotypic Evaluation of BM and PBL Cell Types in WT and Nmp4-KO Mice Using Fluorescence Activated Cell Sorting Analysis as Described in the Materials and Method Section
| |
WT |
KO |
2-Way ANOVA P values |
||||
|---|---|---|---|---|---|---|---|
| Units (%) | VEH | PTH | VEH | PTH | Genotype | Treatment | Gene×Treat |
| Pre-B (BM) | 11.6±7.2 | 12.1±7.0 | 7.6±4.7 | 9.7±5.7 | 0.08 | 0.45 | 0.65 |
| Pre-B (PBL) | 17.5±6.5 | 20.0±7.8 | 11.7±6.5 | 16.9±7.6 | 0.04 | 0.06 | 0.52 |
| Immature B (BM) | 5.9±1.3 | 6.3±2.7 | 6.8±3.5 | 6.3±4.4 | 0.64 | 0.95 | 0.65 |
| Immature B (PBL) | 20.7±10.9 | 22.3±8.5 | 25.1±11.3 | 22.8±11.6 | 0.43 | 0.90 | 0.54 |
| Mature B (BM) | 3.5±3.5 | 3.5±3.7 | 3.0±3.0 | 3.5±3.4 | 0.81 | 0.75 | 0.79 |
| Mature B (PBL) | 5.7±3.8 | 5.5±4.8 | 7.2±6.7 | 8.3±6.7 | 0.20 | 0.79 | 0.68 |
| CD4+T (BM) | 1.6±0.39 | 1.4±0.40 | 1.9±0.74 | 2.0±1.0 | 0.03 | 0.73 | 0.36 |
| CD4+ T (PBL) | 15.8±2.5 | 15.6±4.3 | 16.3±5.5 | 17.1±3.7 | 0.41 | 0.79 | 0.68 |
| Myeloid (BM) | 34.5±5.7 | 36.4±4.8 | 32.0±7.6 | 34.1±11.2 | 0.30 | 0.38 | 0.97 |
| Myeloid (PBL) | 6.0±2.5 | 5.3±1.3 | 5.9±2.9 | 7.4±4.4 | 0.24 | 0.65 | 0.22 |
| LSK (BM) | 0.11±0.06 | 0.11±0.06 | 0.12±0.14 | 0.26±0.22 | 0.05 | 0.07 | 0.09 |
| L5K (PBL) | 0.03±0.03 | 0.03±0.03 | 0.03±0.04 | 0.02±0.03 | 0.60 | 0.95 | 0.92 |
Mice were treated with PTH or vehicle for 3 weeks (number of mice/experimental group=11–14). Statistical significance was set at P<0.0l.
LSK, lin-/Scal+/c-Kit+; BM, bone marrow; PBL, peripheral blood.