FIG. 5.

Molecular profiling for the Hh, Fgf, and Wnt pathways in vitro versus in vivo. Relative mRNA expression levels of the Hh pathway (Shh, Gli1, Gli2, and Gli3) and receptors (Smo and Ptch1), Fgf signaling (Fgf2, Fgf8, and Fgfr3) and Wnt signaling (Wnt1 and Wnt5a) in protocol of mouse ES differentiation toward dopaminergic neurons in treated (Tr., purmorphamine+Fgf2+Fgf8) and untreated (Untr.) experimental conditions of mouse ES differentiation and in the mouse VM at embryonic (E) day 11.5, E12.5, and E13.5. Mean±SEM; n=3–4; *P<0.05; **P<0.01 significantly different from stage V untreated by Student t-test. ^#P<0.05; ^##P<0.01 significantly different from stage V treated by Student t-test. Hh, Hedgehog.