FIG. 2.

Noggin increases primary iPSC-derived neurosphere generation. (A) The number of primary primitive neurospheres generated following 7 days of culture in SFM + LIF with and without Noggin was evaluated from iPSCs grown on MEFs with and without Noggin pretreatment. The presence of Noggin in the SFM + LIF evoked a significant increase in neurosphere number regardless of Noggin pretreatment. Selected neural (B), pluripotency (C), and endodermal (D) gene markers were examined in the passage 3 pNSCs cultured without Noggin (No Noggin), with a single passage with Noggin (1×Noggin), or with continuous Noggin treatment (3×Noggin). No significant differences were detected. C5-4A iPSC line. ND denotes not detected; mean±SEM; n=3, *P<0.05. SFM, serum-free media; pNSC, primitive NSC.