FIG. 5.

In vitro differentiation pattern of iPS-dNSCs(+DLL4) 1 week following plating with 1% fetal bovine serum shows greater neural differentiation compared to iPS-dNSCs(−DLL4). (A) Immunofluorescence images of neural markers (GFAP; astrocytes. Olig2; oligodendrocytes and precursors. PDGFrα; immature oligodendrocytes. βIII-tublin; neurons. Nestin; NSCs). (B) Cell counting for immunofluorescent-positive cell shows the differentiation pattern for iPS-dNSCs cultured±DLL4. iPS-dNSC(+DLL4) yielded predominantly terminally differentiated neural cells, specifically astrocytes, while the majority of iPS-dNSC differentiated cells remained Nestin-positive or were undefined (C5-4A iPSC line) mean±SEM; *P<0.05 scale bar represents 50 μm.