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. Author manuscript; available in PMC: 2013 Jan 21.
Published in final edited form as: Methods Mol Biol. 2012;796:133–174. doi: 10.1007/978-1-61779-334-9_8

Figure 7. Metarhodopsin II Absorption Spectrum.

Figure 7

The absorbance spectra of detergent-solubilized rhodopsin (red) and detergent-solubilized rhodopsin in the presence of Gt (blue) after light activation. In the absence of GTP, Gt significantly increases the meta II signal (380 nm). This likely reflects the stabilization of the activated form of receptor by the heterotrimeric G protein.