Table 2.

Crystallization of G protein α-subunits

	Gαt-GDP(5)	Gαt-GDP-AlF−4(6)	Gαt - GTPγS(4)	Gαi1 -GDP-AlF−4 (31)	Gαi1 - GTPγS (31)
PDB ID	1TAG	1TAD	1TND	1GFI	1GIL
Space Group	I222	P21	P21	P3221	P3221
Resolution	1.8 Å	1.7 Å	2.2 Å	2.2 Å	2.3 Å
Method	Hanging Drop	Batch	Hanging Drop	Sitting Drop	Sitting Drop
Protein Concentration	13.3 mg/m	20 mg/mL	20–25 mg/mL	25–30 mg/mL	25–30 mg/mL
Drop Size	3–5 μL	20 μL	3–5 μL	20 μL	20 μL
Source	Bovine ROS	Bovine ROS	Bovine ROS	Heterologous expression in E. coli	Heterologous expression in E. coli
Protein Buffer	10 mM Tris-Cl pH 7.5, 0.5 mM MgCl2, 50 μM AlCl3, 15 mM NaF, 10% gycerol, 100–200 mM NaCl, 0.1% βME	10 mM Tris-Cl pH 7.5, 0.5 mM MgCl2, 50 μM AlCl3, 15 mM NaF, 10%> glycerol, 100–200 mM NaCl, 0.1% βME	5 mM Tris-Cl pH 7.5, 10 mM MgCl2, 25 mM βME, 40% glycerol	300 μM AlCl3, 5 mM NaF, 5 mM MgCl2, 10 mM DTT, 200 mM sodium acetate pH 6.0	300 μM GTPγS, 5 mM NaF, 5 mM MgCl2, 10 mM DTT, 200 mM sodium acetate pH 6.0
Reservoir Soultion	9% PEG 8000, 10% glycerol, 50 mM MES pH 7.5, 200 mM MgCl2, 0.1% βME	20% PEG 8000, 200 mM CaCl2, 100 mM sodium cacodylate pH 6.0, 0.2% βME, 5 mM MgCl2, 100 μM AlCl3, 30 mM NaF, 40% glycerol	5% PEG 8000, 250 mM sodium cacodylate pH 6.0, 350 mM CaCl2, 25 mM βME, 30% glycerol	1.8–1.9 M ammonium sulfite	1.8–1.9 M ammonium sulfite
Crystallization Temperature	4°C	4°C	−12.5°C	21°C	21°C