Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Jan 14;210(1):125–142. doi: 10.1084/jem.20120525

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Andrews et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 1. — VH gene diversity in LC transgenic mice. (A) Diagram of κ loci in Igκ^Vκ4/h and Igκ^αhel/h knockin mice. (B) VH gene usage of edited and unedited AA4⁻ mature cells sorted from Igκ^Vκ4/h and Igκ^αhel/h mice. Analysis of 141 HC sequences from five Igκ^Vκ4/h mice and 96 HC sequences from five Igκ^αhel/h mice is shown. ANOVA indicated no significant difference between the VH gene repertoires of Vκ4 and αHELκ mice. Error bars indicate SD of the mean. (C) Frequencies of V_H-J_H2 rearrangements. B cells from 12-wk-old mice were sorted into different fractions based on developmental stage (as specified in Fig. S1) and LC cell surface phenotype (where applicable). Genomic DNA from each cell fraction was analyzed by CDR3 spectratyping, and the numbers of peaks were counted per 1,000 cells for V_H J606.1–J_H2 (left) and J558.85–J_H2 (right). Mean VH peak numbers with ± SEM are shown for BM B220⁺CD43⁻IgM⁻pre-B (BP⁺ vs. BP⁻) and mCκ versus hCκ immature (Imm), splenic transitional (Tr), and FM B cells from Igκ^Vκ4/h (closed bars) and Igκ^αhel/h (open bars) mice. Shown are data from cells sorted from two individual mice for each genotype in two independent experiments. (D) Similar frequencies of V_H J606.1-J_H2 rearrangements in B6, Igκ^Vκ4/h, and Igκ^αhel/h. VH peak number analysis in pre-B, Imm, Tr, and FM of B6, Igκ^Vκ4/h, and Igκ^αhel/h mice is shown. The same data for Igκ^Vκ4/h and Igκ^αhel/h mice from panel B are replotted here, but rearrangements per 1,000 sorted B cells were calculated by combining both the sorted mCκ and hCκ populations to facilitate comparison with wild-type B6 mice (which have two mouse κ alleles). Plotted are the frequencies of V_H J606.1–J_H2 with mean ± SEM in both Igκ^Vκ4/h and Igκ^αhel/h mice. No SEM is given for B6 because the B6 analysis was performed on two pooled 12-wk-old mice. (E) V_H CDR3 length distribution comparison in B subsets of Igκ^Vκ4/h and Igκ^αhel/h mice. The CDR3 lengths (nt) of V_H-J_H2 rearrangements in genomic DNA of sorted cell populations were calculated based on CDR3 spectratyping performed in B and C. Each dot represents a single rearrangement. Error bars indicate SEM.