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. 2012 Dec 17;4(1):23. doi: 10.1186/1868-7083-4-23

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Copyright ©2013 Gill-Sharma et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Representative electrophoretogram of rat testis showing bands of ubiquitylated histones and enzymatic molecules implicated in histone ubiquitination during chromatin remodelling. (A) H2AUb. (B) H2BUb. (C) UbHistones probed with Ubiquitin antibody. (D) UbHistones probed with FK1 antibody. (E) UBE1. (F) UBE2D2. (G) URE-B1/E3. (H) GC3α. (I) Proα1. (J) Proα5. (K) Proα7. Lane M: Rainbow markers. Lane F: FD-treated samples. Lane C: Control samples. Lane P: CPA-treated samples. Lane N: Negative controls. L: HeLa cell lysate (Positive control).