Table 3.
Details of PCR primers used for molecular genetic studies
| Rat genes | PCR product (bp) | PCR primers | Gene ID/Accession /GenBank number |
|---|---|---|---|
|
ube1x |
|
Left primer: 5′-GAG-AGG-AAA-TGG-TTC-TCA-CAG-ATT-3′ |
|
| 363 |
Right primer: 5′-AAT-ACT-TGG-CAA-AGT-CAG-TCA-TCA-3′ |
314432 |
|
|
E217Kb/ |
|
Left primer: 5′-CAC-AGT-GGT-CTC-CAG-CAC-TAA-CTA-3′ |
|
|
ube2d2 |
379 |
Right primer: 5′-GCT-AGG-AGA-CAG-TGT-TGG-TAC-AGA-3′ |
U56407 |
|
huwe1 mouse fragment (ure-b1/e3) |
|
Left primer: 5′-GGA-GTA-TGT-GAA-GTT-CGT-GAC-CTC-3′ |
|
| 365 |
Right primer: 5′-GTA-CCT-GTG-ACA-AAC-TGG-AGG-AAC-3′ |
59026 |
|
|
hdac6 |
|
Left primer: 5′-ACA-ACT-GCA-CAG-TGT-GCT-TCAG-3′ |
|
| 417 |
Right primer: 5′-ACA-AGG-TTG-GGA-CAC-ATC-TAGG-3′ |
84581 |
|
|
ubB |
|
Left primer: 5′-GTT-TGT-TCC-TTC-ATC-GCA-TTC-3′ |
|
| 238 |
Right primer: 5′-GTG-CAG-GGT-TGA-CTC-TTT-CTG-3′ |
192255 |
|
|
cdyl |
|
Left primer: 5′-CTA-CCT-AAG-AGC-ACT-CAC-CTG-AAG-3′ |
|
| 344 |
Right primer: 5′-ACA-CTT-GAT-CGG-GAT-CTG-AGAC-3′ |
361237 |
|
|
brdt |
|
Left primer: 5′-CTC-AAG-CTG-CCT-GAC-TAT-TAC-ACC-3′ |
|
| 382 |
Right primer: 5′-GAG-AGA-GAC-AGA-CAT-CAG-GAA-ACC-3′ |
XM_573544 |
|
|
hdac1 |
|
Left primer: 5′-GAT-GTT-TCA-GCC-TAG-TGC-AGTG-3′ |
|
| 162 |
Right primer: 5′-GTA-TAG-CCA-TCT-CCT-CCC-AACA-3′ |
AF321129 |
|
|
miwiI/ |
|
Left primer: 5′-GAG-AGG-TTA-CAA-CCC-AAG-ACT-GAC-3′ |
|
|
piwiLi1 |
400 |
Right primer: 5′-GAG-GTA-GTA-GAG-ACG-GTT-GGA-CAG-3′ |
363912 |
|
mbd2 |
|
Left primer: 5′-GGC-AAG-AGC-GAT-GTC-TAC-TAC-TTC-3′ |
|
| 357 |
Right primer: 5′-GCT-AAG-TCC-TTG-TAG-CCT-CTT-CTC-3′ |
680172 |
|
|
pem(rhoX) |
|
Left primer: 5′-GAG-TGT-CAA-GTC-TGA-GGA-TAA-GCA-3′ |
|
| 187 |
Right primer:5′-GTA-TGC-AGC-CCT-CCT-AAT-CTT-AAA-3′ |
NM_022175 |
|
|
cremΔC-G |
|
Left primer: 5′-AGT-CTG-TAC-AGT-CCC-CAG-CAA-CTA-3′ |
|
|
variant |
322 |
Right primer: 5′-GCA-AGG-GTT-AAG-AGA-CCC-ATC-TAC-3′ |
U048351 |
|
h2a somatic |
|
Left primer: 5′-GAA-TAC-CTG-ACT-GCT-GAG-ATC-CTG-3′ |
|
| 320 |
Right primer: 5′-ACA-GGC-TCA-GTG-TAC-AGC-ACT-TC-3′ |
64646 |
|
|
h2b somatic |
|
Left primer: 5′-AGA-AGA-AGG-ACG-GCA-AGG-AAC-3′ |
|
| 113 |
Right primer: 5′-CAT-GGC-CTT-GGA-AGA-GAT-TC-3′ |
64647 |
|
|
h3 somatic |
|
Left primer: 5′-TCT-CAT-GAT-GCA-TGT-TTC-TGT-ATG-3′ |
|
| 345 |
Right primer: 5′-CAC-ACA-CCT-TAA-TGA-CAA-GAC-TCC-3′ |
117056 |
|
|
th2a |
|
Left primer: 5′-CTC-TTT-CAG-AGC-AGG-TTT-GCAG-3′ |
|
| 320 |
Right primer: 5′-ACT-TGG-TCT-GGG-ACT-TGT-GGT-3′ |
24828 |
|
|
th2b |
|
Left primer: 5′-AGT-CAC-CAA-GAC-CCA-GAA-GAAG-3′ |
|
| 113 |
Right primer: 5′-CTC-GAA-GAT-GTC-TGT-CAC-GAAG-3′ |
24829 |
|
|
h3t |
|
Left primer: 5′-ACG-TGA-AAT-CGC-TCA-GGA-CT-3′ |
|
| 196 |
Right primer: 5′-TTA-TGC-TCT-CTC-CCC-TCG-AA-3′ |
682330 |
|
|
h4 |
|
Left primer: 5′-AGG-AGC-CCA-GAG-TTT-GTT-ACAC-3′ |
|
| 399 |
Right primer : 5′-CTG-GGA-GCA-ATC-TAG-AAG-GATG-3′ |
64627 |
|
| Mouse β actin | |
Left primer: 5′-CTG-GCA-CCA-CAC-CTT-CTA-3′ |
|
| 238 | Right primer: 5′-GGG-CAC-AGT-GTG-GGT-GAC-3′ | 007393(44) |