Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1976 Oct;20(1):196–202. doi: 10.1128/jvi.20.1.196-202.1976

In Vitro Synthesis of Turnip Yellow Mosaic Virus Coat Protein in a Wheat Germ Cell-Free System

C Benicourt 1, A L Haenni 1
PMCID: PMC354980  PMID: 16789166

Abstract

Turnip Yellow Mosaic Virus RNA directs the synthesis in vitro of its coat protein in a wheat germ cell-free extract. Optimum conditions for synthesis have been defined, and the effect of spermine on specifically enhancing coat protein formation has been examined. Identity between the in vitro synthesized coat protein and authentic coat protein of Turnip Yellow Mosaic Virus was established by analysis on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, peptide mapping, and immunoprecipitation.

Full text

PDF
196

Images in this article

199Image
on p.199
199Image
on p.199
200Image
on p.200

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blackburn S. The determination of amino acids by high-voltage paper electrophoresis. Methods Biochem Anal. 1965;13:1–45. doi: 10.1002/9780470110317.ch1. [DOI] [PubMed] [Google Scholar]
  2. Blumenthal T., Landers T. A., Weber K. Bacteriophage Q replicase contains the protein biosynthesis elongation factors EF Tu and EF Ts. Proc Natl Acad Sci U S A. 1972 May;69(5):1313–1317. doi: 10.1073/pnas.69.5.1313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Crawford L. V., Gesteland R. F. Synthesis of polyoma proteins in vitro. J Mol Biol. 1973 Mar 15;74(4):627–634. doi: 10.1016/0022-2836(73)90053-3. [DOI] [PubMed] [Google Scholar]
  4. Davies J. W., Kaesberg P. Translation of virus mRNA: protein synthesis directed by several virus RNAs in a cell-free extract from wheat germ. J Gen Virol. 1974 Oct;25(1):11–20. doi: 10.1099/0022-1317-25-1-11. [DOI] [PubMed] [Google Scholar]
  5. Davies J. W., Kaesberg P. Translation of virus mRNA: synthesis of bacteriophage Q beta proteins in a cell-free extract from wheat embryo. J Virol. 1973 Dec;12(6):1434–1441. doi: 10.1128/jvi.12.6.1434-1441.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Efron D., Marcus A. Translation of TMV-RNA in a cell-free wheat embryo system. Virology. 1973 Jun;53(2):343–348. doi: 10.1016/0042-6822(73)90212-2. [DOI] [PubMed] [Google Scholar]
  7. FRAENKEL-CONRAT H. Degradation of tobacco mosaic virus with acetic acid. Virology. 1957 Aug;4(1):1–4. doi: 10.1016/0042-6822(57)90038-7. [DOI] [PubMed] [Google Scholar]
  8. Fukuma I., Cohen S. S. Polyamines in bacteriophage R17 and its RNA. J Virol. 1975 Aug;16(2):222–227. doi: 10.1128/jvi.16.2.222-227.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. GIERER A., SCHRAMM G. Infectivity of ribonucleic acid from tobacco mosaic virus. Nature. 1956 Apr 14;177(4511):702–703. doi: 10.1038/177702a0. [DOI] [PubMed] [Google Scholar]
  10. Hunter T. R., Hunt T., Knowland J., Zimmern D. Messenger RNA for the coat protein of tobacco mosaic virus. Nature. 1976 Apr 29;260(5554):759–764. doi: 10.1038/260759a0. [DOI] [PubMed] [Google Scholar]
  11. Kamen R., Kondo M., Römer W., Weissmann C. Reconstitution of Q replicase lacking subunit with protein-synthesis-interference factor i. Eur J Biochem. 1972 Nov 21;31(1):44–51. doi: 10.1111/j.1432-1033.1972.tb02498.x. [DOI] [PubMed] [Google Scholar]
  12. Kerr I. M., Martin E. M. Virus protein synthesis in animal cell-free systems: nature of the products synthesized in resonse to ribonucleic acid of encephalomyocarditis virus. J Virol. 1971 Apr;7(4):438–447. doi: 10.1128/jvi.7.4.438-447.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Klein W. H., Nolan C., Lazar J. M., Clark J. M., Jr Translation of satellite tobacco necrosis virus ribonucleic acid. I. Characterization of in vitro procaryotic and eucaryotic translation products. Biochemistry. 1972 May 23;11(11):2009–2014. doi: 10.1021/bi00761a003. [DOI] [PubMed] [Google Scholar]
  14. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  15. Leberman R. The isolation of plant viruses by means of "simple" coacervates. Virology. 1966 Nov;30(3):341–347. doi: 10.1016/0042-6822(66)90112-7. [DOI] [PubMed] [Google Scholar]
  16. Litvak S., Tarragó A., Tarragó-Litvak L., Allende J. E. Elongation factor-viral genome interaction dependent on the aminoacylation of TYMV and TMV RNAs. Nat New Biol. 1973 Jan 17;241(107):88–90. doi: 10.1038/newbio241088a0. [DOI] [PubMed] [Google Scholar]
  17. Marcu K., Dudock B. Characterization of a highly efficient protein synthesizing system derived from commercial wheat germ. Nucleic Acids Res. 1974 Nov;1(11):1385–1397. doi: 10.1093/nar/1.11.1385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Marcus A. Tobacco mosaic virus ribonucleic acid-dependent amino acid incorporation in a wheat embryo system in vitro. Analysis of the rate-limiting reaction. J Biol Chem. 1970 Mar 10;245(5):955–961. [PubMed] [Google Scholar]
  19. Peter R., Stehelin D., Reinbolt J., Collot D., Duranton H. Primary structure of turnip yellow mosaic virus coat protein. Virology. 1972 Aug;49(2):615–617. doi: 10.1016/0042-6822(72)90516-8. [DOI] [PubMed] [Google Scholar]
  20. Roberts B. E., Gorecki M., Mulligan R. C., Danna K. J., Rozenblatt S., Rich A. Simian virus 40 DNA directs synthesis of authentic viral polypeptides in a linked transcription-translation cell-free system. Proc Natl Acad Sci U S A. 1975 May;72(5):1922–1926. doi: 10.1073/pnas.72.5.1922. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Roberts B. E., Paterson B. M. Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2330–2334. doi: 10.1073/pnas.70.8.2330. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Rolleston F. S., Wool I. G., Martin T. E. Binding and translation of turnip-yellow-mosaic-virus ribonucleic acid by skeletal-muscle ribosomes from normal and diabetic rats. Biochem J. 1970 May;117(5):899–905. doi: 10.1042/bj1170899. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Schweiger M., Gold L. M. Bacteriophage T4 DNA-dependent in vitro synthesis of lysozyme. Proc Natl Acad Sci U S A. 1969 Aug;63(4):1351–1358. doi: 10.1073/pnas.63.4.1351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Schwinghamer M. W., Symons R. H. Fractionation of cucumber mosaic virus RNA and its translation in a wheat embryo cell-free system. Virology. 1975 Jan;63(1):252–262. doi: 10.1016/0042-6822(75)90389-x. [DOI] [PubMed] [Google Scholar]
  25. Shih D. S., Kaesberg P. Translation of brome mosaic viral ribonucleic acid in a cell-free system derived from wheat embryo. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1799–1803. doi: 10.1073/pnas.70.6.1799. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Shih D. S., Kaesberg P. Translation of the RNAs of brome mosaic virus: the monocistronic nature of RNA1 and RNA2. J Mol Biol. 1976 May 5;103(1):77–88. doi: 10.1016/0022-2836(76)90053-x. [DOI] [PubMed] [Google Scholar]
  27. Takeda Y., Onishi T. Binding of transfer RNA to polyamines in preference to Mg-2+. Biochem Biophys Res Commun. 1975 Apr 7;63(3):611–617. doi: 10.1016/s0006-291x(75)80428-1. [DOI] [PubMed] [Google Scholar]
  28. VOORMA H. O., GOUT P. W., VAN DUIN, HOOGENDAM B. W., BOSCH L. THE READ-OUT OF TURNIP YELLOW MOSAIC VIRUS RIBONUCLEIC ACID AS A POLYCISTRONIC MESSENGER IN CELL-FREE SYSTEMS OF ESCHERICHIA COLI. Biochim Biophys Acta. 1965 Mar 15;95:446–460. doi: 10.1016/0005-2787(65)90191-7. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES