Table 1.
List of primers and TaqMan probes for each targeted oral species with expected amplicon sizes
| Primers or probesa, b | Sequence (5′➞3′) | Product size (bp) | Source |
|---|---|---|---|
|
S. oralis |
|
|
|
| gtfR F |
ACCAGCAGATACGAAAGAAGCAT |
235 |
This study |
| gtfR R |
AGGTTCGGGCAAGCGATCTTTCT |
|
|
| TaqMan probe |
AAGGCTGCTGTTGCTGAAGAAGT |
|
|
|
S. gordonii |
|
|
|
| gtfG F |
CGGATGATGCTAATCAAGTGACC |
177 |
This study |
| gtfG R |
GTTAGCTGTTGGATTGGTTGCC |
|
|
| TaqMan probe |
AGAACAGTCCGCTGTTCAGAGCAA |
|
|
|
V. parvula |
|
|
|
| 16S rRNA F |
GGATAGATGAAAGGTGGCCTCT |
72 |
This study |
| 16S rRNA R |
CCAACTAGCTAATCAGACGCAAT |
|
|
| LNA probe* |
FAM-GAAGGAGG |
|
|
|
F. nucleatum |
|
|
|
| fadA F |
TGCAGCAAGTTTAGTAGGTG |
146 |
This study |
| fadA R |
CATTGTAAACTTGTTCATTTTGT |
|
|
| TaqMan probe |
AGCACTAGATGCTGAATACCAA |
|
|
|
P. intermedia |
|
|
|
| 16S rRNA F |
CGGTCTGTTAAGCGTGTTGTG |
100 |
Loozen et al. |
| 16S rRNA R |
CACCATGAATTCCGCATACG |
|
|
| TaqMan probe | TGGCGGACTTGAGTGCACGC |
a) F, Forward primer; R, Reverse primer.
b) All TaqMan probes correspond to: 5′ FAM, 3′ TAMRA.
* LNA probe, Roche Diagnostics.