Table 1.
Results of S-S bond determination on a set of Glycosyltransferases using MS-based and sequence-based methods.
| Swiss-Prot ID | Known S-S bonds | Methods that detected the bond | Methodology |
|---|---|---|---|
| Q92187 | 142-292 | MassMatrix | MS-based |
| 156-356 | MassMatrix | MS-based | |
| P02754 | 82-176 | MassMatrix, DisLocate, DiANNA 1.1 | MS-based, Sequence-based |
| 122-135 | - | - | |
| Q11130 | 68-176 | MassMatrix, PreCys | MS-based, Sequence-based (cysteine separation profile) |
| 211-214 | MassMatrix, PreCys | As above | |
| 318-321 | MassMatrix, PreCys | As above | |
| P08037 | 134-176 | MassMatrix | MS-based |
| 247-266 | MassMatrix | MS-based | |
| Q09324 | 59-413 | - | - |
| 100-172 | - | - | |
| 151-199 | - | - | |
| 372-381 | - | - | |
| P00698 | 24-145 | DISULFIND, DiANNA 1.1 | Sequence-based |
| 48-133 | MassMatrix, DiANNA 1.1, DISULFIND | MS-based, Sequence-based | |
| P21217 | 81-338 | - | - |
| 91-341 | - | - |
The molecules and their Swiss-Prot ID are: ST8Sia IV [Swiss-Prot:Q92187], Beta-lactoglobulin [Swiss-Prot:P02754], FucT VII [Swiss-Prot:Q11130], C2GnT-I [Swiss-Prot:Q09324], Lysozyme [Swiss-Prot:P00698], FT III [Swiss-Prot:P21217], β1-4GalT [Swiss-Prot:P08037], Aldolase [Swiss-Prot:P00883], and Aspa [Swiss-Prot:Q9R1T5]. The methods investigated include MassMatrix [21], DisLocate [24], DISULFIND [14], PreCys [4], and DiANNA 1.1 [17].