Figure 2. Type I collagen fibrils from bovine hide prepared in vitro. (A) Collagen without additives. Note that fibrils of bovine origin are generally thicker than those shown in Figure 1. (B) Collagen with 10 µg decorin per 100 μg collagen. Addition of decorin results in increasingly finer fibrils. (C) Collagen with 2 µg chondroitin sulfate A per 100 μg collagen. Lower amounts of CS with a lower sulfation degree (~0.9) result in fibrils that are larger than collagen without additives, compared with the higher amount and sulfation degree as shown in Figure 1. Images 5 x 5 µm, AFM tapping mode (the authors thank Dr. S. Heinemann for the AFM-measurement).