Table 1. PCR and qRT-PCR primers.
| Primer name | Forward primer (5'–3') | Reverse primer (5'–3') |
|---|---|---|
|
Real-time RT-PCR |
|
|
| NKG2D |
CACAGCTGGGAGATGAGTGA |
TCGGTCAAGGGAATTTGAAC |
| DAP10 |
GGCACTTCAGGCTCTTGTTC |
CCAGGATGAGAGGGTCAGAA |
| PRF1 |
ACTCACAGGCAGCCAACTTT |
GGGTGCCGTAGTTGGAGATA |
| GZMB |
ACTGCAGCTGGAGAGAAAGG |
TTCGCACTTTCGATCTTCCT |
| GAPDH |
ACCACAGTCCATGCCATCAC |
TCCACCACCCTGTTGCTGTA |
|
DNA methylation |
|
|
| NKG2D Amp 1 |
GATGTTTGAATAGAATTTTTGAGG |
AATTTTCTTTCTACAAACTAAACTCAA |
| NKG2D Amp 2 |
TTGGAAATATTTGATTTTAGTTAAA |
CTTCAATACACAAAAAATTCCT |
| NKG2D Amp 3 |
GGAATTTTTTGTGTATTGAAGA |
ACACAAAAATTACAAATTAACCC |
| DAP10 Amp 1 |
GTTTAGGTTGGAGTGTAGTGGT |
ACAAAATTCTACCCCTATCACA |
| DAP10 Amp 2 |
TGTTGTGATAGGGGTAGAATTT |
TCCTAAACAACCAAACCTTCTA |
| DAP10 Amp 3 |
TAGAAGGTTTGGTTGTTTAGGA |
CCAAAAAAATCCAAAAAAACT |
|
ChIP |
|
|
| NKG2D Amp1 |
GCACAGGGGAAAAGTTTCTG |
ACGTCTACCGCAGAGAGGAA |
| NKG2D Amp 2 | GGCCAAGAAGCAATAAACGA | GCAGAAACTTTTCCCCTGTG |