Figure 4. The adhesive effect of FSP was not affected by the FLAG tag.

FSP treated with (+) or without (−) enterokinase (EK) was resolved by SDS-PAGE under non-reducing conditions and was immunoblotted with anti-FLAG IgG or anti-SPARC IgG (A). FSP (10 ug/ml) with(+) or without (−) EK was coated onto 96-well plates overnight at 4°C. LEC were plated into the wells and incubated in serum-free DMEM for 2 hr at 37°C. Cells were stained with crystal violet (B); cell attachment was also quantified by a colorimetric assay at 562 nm (C). Results are mean +/− SD of experiments carried out in triplicate. NS, not significant (P>0.05).