Table 1. Primers used for nested PCR.
Primer | Region | Product size | Nucleotides |
Forward:5′CGTGAGGGCATCGAGGTGGC3′ | INS | 245 bp | 631–650 |
Reverse:5GCGTAGGCGTCGGTGACAAA3′ | 856–875 | ||
Forward:5′CTCGTCCAGCGCCGCTTCGG3′ | IS | 123 bp | 762–781 |
Reverse:5′CCTGCGAGCGTAGGCGTCGG3′ | 865–884 | ||
Forward:5′TGAACGTGGATGAAGTTGGTGGTG3′ | β-globin | 291 bp | - |
Reverse:5′ACTTTCTTGCCATGAGCCTTCACCTT3 |