Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Dec 28;8:248. doi: 10.1186/1746-6148-8-248

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright ©2012 Lee et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Examination of the specificity of the two polyclonal antibodies against canine NGAL by Western blotting. (A) The recombinant NGAL protein was separated by SDS-PAGE and processed for Western blot analysis. As indicated by the arrowhead, the positive control antibody (+; antibody against histidine) as well as the sera of the mice (lane 2) and the rabbit (lane 4) immunized with NGAL were able to recognize the recombinant canine NGAL protein (~42 kDa), but the pre-immunized sera of mouse (lane 1) and rabbit (lane 3) failed to detect recombinant NGAL protein. (B) Three clinic urine samples collected from one healthy dog (lane 1) and two renal failure dogs (lane 2 and 3) were used to examine the specificity of serum obtained from mice and rabbits immunized with recombinant NGAL. Both rabbit and mouse sera were able to recognize the endogenous NGAL (~25 kDa) as indicated by the arrowheads.