Table 1. Chemical shift data (ppm) for anomeric resonances from proton NMR spectra at 600 MHz of V. cholerae binding glycosphingolipids, and co-migrating glycosphingolipids, obtained in DMSO-d6/D2O (98∶2, by volume) at 30°C.
X | IX | VIII | VII | VI | V | IV | III | II | I | Fraction | ||
A | Galα3 | Galβ4 | GlcNAcβ3 | Galβ4 | Glcβ1 | Cer | TH-II, RE-I | |||||
4.826 | 4.279 | 4.627 | 4.25 | 4.16 | ||||||||
B | GlcNAcβ3 | Galβ4 | GlcNAcβ3 | Galβ4 | Glcβ1 | Cer | TH-I | |||||
4.604 | 4.250 | 4.640 | 4.250 | 4.15 | ||||||||
C | Galβ4 | GlcNAcβ3 | Galβ4 | GlcNAcβ3 | Galβ4 | Glcβ1 | Cer | TH-II | ||||
4.20 | 5.643 | 4.253 | 4.643 | 4.253 | 4.16 | |||||||
D | Galα3 | Galα3 | Galβ4 | GlcNAcβ3 | Galβ4 | Glcβ1 | Cer | RE-I | ||||
4.826 | 4.893 | 4.28 | 4.66 | 4.25 | 4.16 | |||||||
E | Galβ4 | GlcNAcβ3 | Galβ4 | GlcNAcβ3 | Galβ4 | Glcβ1 | Cer | TH-III | ||||
4.13 | 4.796 | 4.25 | 4.64 | 4.25 | 4.16 | |||||||
F | Galα3 | Galβ4 | GlcNAcβ3 | Galβ4 | GlcNAcβ3 | Galβ4 | Glcβ1 | Cer | TH-III, IV | |||
4.826 | 4.279 | 4.626 | 4.257 | 4.78 | 4.257 | 4.16 | ||||||
G | Galβ3 | (Fucα4) | GlcNAcβ3 | Galβ4 | Glcβ1 | Cer | HI-I | |||||
4.293 | 4.767 | 4.727 | 4.260 | 4.207 | ||||||||
H | Galβ3 | GlcNAcβ3 | Galβ3 | (Fucα4) | GlcNAcβ3 | Galβ4 | Glcβ1 | Cer | HI-I | |||
4.14 | 4.794 | 4.358 | 4.77 | 4.712 | 4.26 | 4.21 | ||||||
I | Galβ3 | (Fucα4) | GlcNAcβ3 | Galβ3 | (Fucα4) | GlcNAcβ3 | Galβ4 | Glcβ1 | Cer | HI-I | ||
4.309 | 4.77 | 4.76 | 4.358 | 4.77 | 4.712 | 4.26 | 4.21 | |||||
J | Galβ4 | GlcNAcβ3 | Galβ4 | GlcNAcβ3 | Galβ4 | GlcNAcβ3 | Galβ4 | Glcβ1 | Cer | TH-IV | ||
4.209 | 4.651 | 4.255 | 4.651 | 4.255 | 4.651 | 4.255 | 4.16 | |||||
K | Galα3 | Galβ4 | GlcNAcβ6 | (Galα3 | Galβ4 | GlcNAcβ3) | Galβ4 | GlcNAcβ3 | Galβ4 | Glcβ1 | Cer | DE-I |
4.827 | 4.282 | 4.395 | 4.827 | 4.282 | 4.652 | 4.290 | 4.652 | 4.254 | 4.161 |