Table 2. Saccharomyces cerevisiae gene deletion mutants used in this study.
Strain a | Genotype | Description b | PAF26 susceptibility c |
BY4741 | MATa his3Δ1 leu2Δ0 met15Δ0 uraΔ0 | Parental Strain | |
Y04007 | Same as BY4741 ipt1Δ::KanMX4 | Inositolphosphotransferase, involved in synthesis of the sphingolipid mannose-(inositol-P)2-ceramide [M(IP)2C] | R |
Y01750 | Same as BY4741 arg1Δ::KanMX4 | Arginosuccinate synthetase (arginine biosynthesis and urea cycle). Cytosolic. | R |
Y03215 | Same as BY4741 ecm33Δ::KanMX4 | GPI-anchored protein of unknown function. Cell wall/plasma membrane. | S |
Strain name at the EUROSCARF collection (http://web.uni-frankfurt.de/fb15/mikro/euroscarf/index.html).
Summary of annotations found at the Saccharomyces Genome Database (http://www.yeastgenome.org/).
Susceptibility of each strain to PAF26, as reported in [24]. “R” stands for increased resistance and “S” for increased susceptibility, respectively, as compared to the parental strain BY4741.