Figure 1. An ALP activity-based high-throughput screening assay of chemical library for PKC inhibitors.

The ALP activity using 4-methylumbelliferyl phosphate [59] in 201B7 hiPS cells in a 96-well plate was measured by fluorometry. Each dot on the graph represents the fluorescent intensity for each compound of the kinase inhibitor library. Dotted line indicates the level for DMSO as a control.