Table 1. List of Primers.
No | Product ID | Type | Oligo sequence (5′-3′) | |
P1 | MUC2F1 | RT | F | TTT ATG CTC TGG CTG GCT CTT T |
P2 | RT | R | GGA GTC CTC ATT TCC TTT ACA TGC | |
P3 | MUC2F2 | RT | F | ATT GTC ACT CAC GCC TTA ATC TG |
P4 | RT | R | TTT GTC ATC TAC TAA CAA CAC AAC AGT C | |
P5 | MUC2F3 | RT | F | ATG TGG TGG TTT TCA GAT CAG ATG |
P6 | RT | R | AGG TTC CAG ATA TGA CCC CTT GTA | |
P7 | MUC2F4 | RT | F | TGT GGC TGC CCA GAT AAT ACA TAC |
P8 | RT | R | CCA TTC CTG CTT GTA AAG TCA TTG | |
P9 | MUC2F5 | RT | F | TGA TGT GCA TTA CCA GAA CAA GAC |
P10 | RT | R | ATA TGT CGC CAT CCT TTA TTG TTG | |
P11 | MUC2F6 | RT | F | TGG TGG AGA AAT ACC AAC AGA AGA |
P12 | RT | R | TAT TGG TGG TAG GAC TGT GCT TGT | |
P13 | MUC2F7 | RT | F | CCA CCA CAA GCC AGT CTC CA |
P14 | RT | R | GCA GTA TGA AAC ATG GCC GTT G | |
P15 | MUC2F8 | RT | F | TGA AGA ATT AGG GCA GAA GGT TGA |
P16 | RT | R | GGG CAC TGC TAC TTG ACA CAG TC | |
P17 | MUC2F9 | RT | F | AAC ACG TAC GAC TGT GTC AAG TAG C |
P18 | RT | R | GCT GTT GTG CAC TCT GGA CTT AAT | |
P19 | MUC2F10 | RT | F | ATT AAG TCC AGA GTG CAC AAC AGC |
P20 | RT | R | GCA CTG CTA CTT GAC ACA GTC GT | |
P21 | MUC2F11 | RT | F | ACA TTC CTA TAG AAG ATC TAG GGC AGA A |
P22 | RT | R | CTC CCT CAA CAG GGG AAC AC | |
P23 | MUC2F12 | RT | F | ACG ACT GTG TCA AGT AGC AGT GC |
P24 | RT | R | TAC TTG ACA CAC TTG GAC TCG ACA | |
P25 | MUC2F13 | RT | F | ACC ACC AGT ACA ACA GTG TCG AGT |
P26 | RT | R | TTC CAC TTT CTG CCC TAG ATC TTC | |
P27 | MUC2F14 | LR | F | ACA CAG TTC ACC CAC CTT AGC C |
P4 | LR | R | TTT GTC ATC TAC TAA CAA CAC AAC AGT C | |
P28 | MUC2F15 | LR | F | AAC GGC AAC TGA AAT AGT CTG CAC CTT C |
P29 | LR | R | AAT GTG CTT TTA ATC ATT CAG AGA AAA TAA GTT GAT T | |
P30 | MUC2F16 | LR | F | CGG GCC AAC ACC TAC CAC CTC |
P29 | LR | R | AAT GTG CTT TTA ATC ATT CAG AGA AAA TAA GTT GAT T | |
P31 | MUC2F17 | LR | F | TAA CTC AAA CCC ACT CTC CTC CAC CTT C |
P29 | LR | R | AAT GTG CTT TTA ATC ATT CAG AGA AAA TAA GTT GAT T | |
P19 | MUC2F18 | LR | F | ATT AAG TCC AGA GTG CAC AAC AGC |
P22 | LR | R | CTC CCT CAA CAG GGG AAC AC | |
P32 | MUC2F19 | 5′RACE | R | CTA ACA CAT GGA AAG CTC AGC CCA CC |
P33 | MUC2F20 | 3′RACE | F | CGG GCC AAC ACC TAC CAC CTC |
P34 | MUC2RQ | qRT | F | ATT GTG GTA ACA CCA ACA TTC ATC |
P35 | qRT | R | CTT TAT AAT GTC AGC ACC AAC TTC TC | |
P36 | 18S | qRT | F | GCC ACC CGA GAT TGA GCA ATA ACA |
P37 | qRT | R | TAG ACA CAA GCT GAG CCA GTC AGT | |
P38 | HPRT | RT-PCR | F | ATG ACC ACT GTC CAT GCC ATC |
P39 | RT-PCR | R | AGG GAT GAC TTT CCC TAC AGC CTT | |
P40 | ARK clone | Seq | F | GGA GAG AGT TGT CCT GAC TGA ATG |
P41 | ARK clone | Seq | R | CAC AAG AGA AGA GCC ATC AG |
P42 | MUC2F15,16,17 | Seq | R | TCC AGG TCT AAG TCG GGA AGT G |
P43 | MUC2F15,16,17 | Seq | F | CAC CTC CTA AAC CCA CCT GCT |
P44 | MUC2F15,16,17 | Seq | R | CCG CAG CTT TCC ACA TAC AC |
P45 | MUC2F15,16,17 | Seq | F | GTG TTT GAG AAG TGC CGT GAA G |
P46 | MUC2F15,17 | Seq | F | ACA CTC AAC CAC TAC AAC CAT |
P47 | MUC2F15,17 | Seq | R | AAG GTA ATT GTC TGG CCG TGG TG |
P48 | MUC2F15 | Seq | F | CCT GTT AAC ACA CAG TCT ACA GGA G |
P49 | MUC2F15 | Seq | F | GCT CTT CAA CAG CTT CAG TTT |
P51 | MUC2F15 | Seq | R | GGC TCA CAG ATT ACT GGA ACG A |
P52 | MUC2F15 | Seq | R | ATT GGA GCA GGT GGG TTT AGG |
P53 | E45F | DNA | F | AGA GCT CTC AGA CAC AGT GGT TGT |
P54 | E46R | DNA | R | CAT TTT CCA TGA GCT CCC TTA CTT |
RT-PCR (RT); long-range PCR (LR); quantitative RT-PCR (qRT); sequencing (Seq); Forward (F); Reverse (R).