Table 2. Genomic Structure of Chicken MUC2.
Exon | cDNA | Exon | UCSC Alignment1 | Intron | Splice Junctions | |||
# | Start | Stop | length | Start | Stop | length | 5′ Splice acceptor | 3′ Splice donor |
1a | 1 | 94 | 94 | 14368918 | 14368825 | 1490 | cagtggtattCACAGTTCAC | ATAAGAAAAGgtaagctcta |
2 | 95 | 359 | 265 | 14367335 | 14367071 | 2588 | aacttaacagGAAGGACAAG | ATGGGCGGATgtaagtacat |
3 | 360 | 474 | 115 | 14364483 | 14364369 | 698 | ttcttcctagTGTCAAGACA | TGCACTGATGgtatgtaaaa |
4 | 475 | 565 | 91 | 14363671 | 14363581 | 411 | cattacctagGTGGAGCTGG | ATTAGTGGAGgtgagtcata |
5 | 566 | 675 | 110 | 14363170 | 14363061 | 237 | tttctcacagTTGCAAGCTA | TAATGAGCATgtgagtaagc |
5b | 566 | 744 | 179 | 14363060 | 14362992 | 168 | taatgagcatGTGAGTAAGC | TGAGATGTTAgtgtgtgaag |
6 | 745 | 961 | 217 | 14362824 | 14362608 | 450 | tccttcccagCGTGAGGAAT | AACTTTTGCTgtaagcagct |
7 | 962 | 1087 | 126 | 14362158 | 14362033 | 260 | tttttttcagACAAAACATG | TGCCCCGAAGgcaagtgtat |
8 | 1088 | 1205 | 118 | 14361773 | 14361656 | 318 | tgctttacagGGACTGTGTA | GTGAAGAATGgtaaggatca |
9 | 1206 | 1344 | 139 | 14361338 | 14361200 | 663 | ttgtttatagCACCTGTGAT | GTTGGCCAAGgtactgtata |
10 | 1345 | 1455 | 111 | 14360537 | 14360427 | 1040 | gtgattgtagAGTGCTGTGA | CAAAAAAAATgtaagtgttg |
11 | 1456 | 1525 | 70 | 14359387 | 14359318 | 1334 | ttttatacagGTGGTGGTTT | CATGTGTCAGgtaaggacca |
12 | 1526 | 1660 | 135 | 14357984 | 14357850 | 527 | tgtttttcagCTAGCTTCTC | GACATTCAAGgcaagtgtcc |
12b | 1526 | 1735 | 210 | 14357849 | 14357774 | 451 | acattcaaggCAAGTGTCCA | ACATGGGTGGtaattcatag |
13 | 1736 | 1900 | 165 | 14357323 | 14357159 | 608 | tttttgatagGTCTTTGTGG | ATTGAAAGTGgtaagcttgc |
14 | 1901 | 1995 | 95 | 14356551 | 14356457 | 623 | ttttttgcagCAAATTATGC | ATACTATAAGgtatggtaat |
15 | 1996 | 2122 | 127 | 14355834 | 14355708 | 578 | gctttttcagAGATGTAAAT | AGTGTCTGCTgtaagtaatg |
16 | 2123 | 2378 | 256 | 14355130 | 14354875 | 854 | tcccctccagCTGATGAAGT | GAGAACGTTGgtatgtgtta |
17 | 2379 | 2431 | 53 | 14354021 | 14353969 | 289 | tctttcctagTGTTTGCCGA | AGAATGAAAAgtaagtgaca |
18 | 2432 | 2541 | 110 | 14353680 | 14353571 | 435 | taactttcagGTATAACAGA | AACTGATCTTgtatgtattc |
19 | 2542 | 2693 | 152 | 14353136 | 14352985 | 433 | tgctccttagTATCAAGGTG | GCAACACCTGgtatgctggt |
20 | 2694 | 2832 | 139 | 14352552 | 14352414 | 543 | tctcttctagTACCTGCCAG | GGCTACTCAGgtaatgctca |
21 | 2833 | 2943 | 111 | 14351871 | 14351761 | 342 | ttatccacagGATTATTGCG | GTTTATAGGGgtgagtaatg |
22 | 2944 | 3123 | 180 | 14351419 | 14351240 | 995 | taccatttagAAAACTGAAC | TGACTATAAAgtaagttaga |
23 | 3124 | 3363 | 240 | 14350245 | 14350006 | 1149 | ccttttgcagGGGAAAGTGT | TCATTCCAAGgtttgtagat |
24 | 3364 | 3520 | 157 | 14348857 | 14348701 | 1075 | tttccaacagGTGAACCCAT | GATATATGCCgtgagtaaca |
25 | 3521 | 3649 | 129 | 14347626 | 14347498 | 1713 | ttctcatcagCAATATTCTG | TACCTGGAAGgtaataaatt |
26 | 3650 | 3797 | 148 | 14345785 | 14345638 | 911 | gcatcaatagGTTGCTACCC | GTACAAAATGgtatgtaaaa |
27 | 3798 | 3841 | 44 | 14344727 | 14344684 | 473 | ttgcttttagTATCTGTCGC | CCAATTCCAGgtaaatagtg |
28 | 3842 | 4039 | 198 | 14344211 | 14344014 | 354 | tttcttgcagGATGTCCTTG | ACCACCATAGgtaagttttc |
29 | 4040 | 4066 | 27 | 14343660 | 14343634 | 350 | tctatttcagTTACCACAAG | GTACCTACAAgtaagttttg |
30 | 4067 | 4543 | 477 | 14343284 | 14342808 | 852 | tttcttgcagCTCCATGCCT | GGCTCCACAGgtatttagca |
31 | 4544 | 4735 | 192 | 14341956 | 14341765 | 1072 | tctcttccagTATCAACCAC | TCGCAACCAGgtgattaatt |
32 | 4736 | 5338 | 603 | 14340693 | 14340091 | 1025 | tgtctctcagTAGGAAATTG | TCGACAGAAGgtaattgtct |
33 | 5339 | 5577 | 239 | 14339066 | 14338828 | ttttccacagGTCCCACTCC | GACAACTGAAggtaatgtct | |
34c | 5578 | 5775 | 198 | nnnnnnnnnnATAGTCTGCA | AGAAGATCTAnnnnnnnnnn | |||
35 | 5776 | 5786 | 11 | 14338698 | 14338688 | tgtttgtcttGGGCA | GAAAGTcccttagtc | |
36d | 5787 | 6209 | 423 | nnnnnnnnnnGGAATGCGAT | CGTCGACAGAnnnnnnnnnn | |||
37 | 6210 | 6453 | 244 | 14336621 | 14336378 | gtgttatcccAGGTCCCACT | GACAGAAGGTaattgtctgg | |
38c | 6454 | 6497 | 44 | nnnnnnnnnnCCCACTTCCC | CGAGTCCAAGnnnnnnnnnn | |||
39e | 6498 | 6675 | 178 | 14335163 | 14334986 | cacgtacgacTGTGTCAAGT | ACCCACCACCnnnnnnnnnn | |
40d | 6676 | 6704 | 29 | nnnnnnnnnnTCCGTAACAC | CGTCGACAGAnnnnnnnnnn | |||
41 | 6705 | 6946 | 242 | 14313408 | 14313167 | 1173 | gtgttatcccAGGTCCCACT | TCGACAGAAGgtaattgtct |
42 | 6947 | 7203 | 257 | 14311994 | 14311738 | gttttcccagGTCCCACTTC | GACAGAAGGTaattgtctgg | |
43c | 7204 | 8072 | 869 | nnnnnnnnnnCCCACTCCTG | CGTCGACAGAnnnnnnnnnn | |||
44 | 8073 | 8359 | 287 | 14310552 | 14310266 | 1463 | gtgttatcccAGGTCCCACT | TTGAGGGAGAggtgaaggcc |
45 | 8360 | 8363 | 4 | 14308803 | 14308800 | gagctcccttAC | TTtgtgcttttt | |
46 | 8364 | 8823 | 460 | 14308740 | 14308281 | 1002 | ttttctcaggAACTTCTTCG | AACTCATGCGgttagtgaat |
47 | 8824 | 8998 | 175 | 14307279 | 14307105 | 566 | ccaaatacagCCGGGTGAGT | GAGTGTGATTgtaagtatat |
48 | 8999 | 9246 | 248 | 14306539 | 14306292 | 888 | gtttttgcagGCTACTGCAC | GGAAGTAGAGgtattggaga |
49 | 9247 | 9430 | 184 | 14305404 | 14305221 | 134 | tatttactagGTGACTGTAA | GGACAGTGTGgtaaggctta |
50 | 9431 | 9635 | 205 | 14305087 | 14304883 | 644 | cccattttagGCATTTGCAA | TTTGGGGAAGgtaggcatgc |
51 | 9636 | 9814 | 179 | 14304239 | 14304061 | 176 | ctgtttttagTGTGTTTGAG | GGTGTTTGCTgtaagtattt |
52f | 9815 | 9817 | 3 | 14303885 | 14303883 | 1879 | ccgctctctgGC | Tgtgtgtgtgg |
53 | 9818 | 9887 | 70 | 14302004 | 14301935 | 315 | cccattccagCTTATGAATG | GCAAGTCAAGgtaaagaatt |
54b , f | 9888 | 9988 | 101 | 14301620 | 14301520 | 157 | tctcatacagTCCACAAAA | AATACCTGTGgtgagtttta |
55 | 9989 | 10020 | 32 | 14301363 | 14301332 | 157 | attttcacagGCTGTGTTGG | ACCAAGAGAGgtacgctgcc |
56 | 10021 | 10198 | 178 | 14301175 | 14300998 | 707 | ttaatgacagTTTGGAGAAA | GTCACTTGCAgtgagttaat |
57 | 10199 | 10303 | 105 | 14300291 | 14300187 | 576 | tttttttcagAATGCAACAC | TACAAGTGTGgtaagtcctt |
58 | 10304 | 10344 | 41 | 14299611 | 14299571 | 644 | ttgttaacagTTCCCAAGAA | TGAGTTCTTGgtaagttaat |
59 | 10345 | 10467 | 123 | 14298927 | 14298805 | 452 | aaatttatagCCTAATTCCT | TTGTGAACCAgtaagtgatg |
60 | 10468 | 10566 | 99 | 14298353 | 14298255 | 625 | taatatctagGGATATGAAC | CATACTGAATgtaagtatgc |
61 | 10567 | 10695 | 129 | 14297630 | 14297502 | 665 | tcacttgcagCCTGGAGAGT | CTGCAAACCTgtaagtagat |
62 | 10696 | 10735 | 40 | 14296837 | 14296798 | 289 | gttcttttagGGAACTGTTA | TGCAAAACCTgtaagtatgt |
63 | 10736 | 10865 | 130 | 14296509 | 14296380 | 1513 | ttccctttagGTATACCTCT | CTTTCTCACTgtaagtaaac |
64g | 10866 | 11359 | 494 | 14294867 | 14294374 | atcttaacagGTACTCTGTT | ATTAAAAAAAaaaacatggg |
Nov 2011 Build (ICGSC Gallus_gallus-4.0/galGal4).
Translational start site at nucleotide 25.
Predicted alternate splicing events (exons 5b & 12b) or exons (exon 55) from reference [3] that were not cloned in this study.
Assembly error—Exon is missing from the current assembly.
Assembly error—Exon located in Gap.
Assembly error—Exon ends in Gap.
Sequence was found in cerebrum and in primordial germ cells in the embyronic gondal, but not cloned in intestine in this study.
Translational stop site at nucleotide 11117.