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. 2013 Jan 21;8(1):e54463. doi: 10.1371/journal.pone.0054463

Figure 8. Systemic injection of garcinol impairs the consolidation and reconsolidation of a fear memory.

Figure 8

(a) Schematic of the behavioral protocol. In the consolidation experiment, rats were fear conditioned with two tone-shock pairings followed 30 min later by i.p. injection of either garcinol (10 mg/kg; n = 8) or vehicle (n = 8). STM was examined 3 hrs later and LTM 21 hrs following injections. In the reconsolidation experiment, rats were fear conditioned with two tone-shock pairings followed 24 hrs later by fear memory reactivation session and i.p. injection of either garcinol (R-Garcinol; n = 9) or vehicle (R-Vehicle; n = 9). A third group received garcinol following a no-reactivation control session (NR-Garcinol; n = 8). All rats received tests of PR-STM and PR-LTM 3 hrs and 21 hrs after injections, respectively. (b) Mean (± SEM) percent freezing during the STM and LTM tests in vehicle and garcinol-infused groups in the consolidation experiment. * p<0.05 relative to vehicle group. (c) Memory retrieval data for the R-Vehicle, R-Garcinol, and NR-Garcinol groups in the reconsolidation experiment. *p<0.05 relative to the pre-CS period. (d) Mean (± SEM) percent freezing during the PR-STM and PR-LTM tests in R-Vehicle, R-Garcinol, and NR-Garcinol groups. *p<0.05 relative to vehicle-infused controls.