Table 1. Quantification of axon branching defects in turnout and plexin A3 mutants.
| turnoutsiblings | turnoutmutants | plexin A3 siblings | plexin A3 mutants | |
| Time of ventral bifurcation | 29.8+/−0.5 hpf (n = 12) | 28.9+/−0.3 hpf (n = 15) | 29.4+/−0.3 hpf (n = 12) | 29.2+/−0.4 hpf (n = 14) |
| Position of ventral bifurcation* | 89.8+/−3.1 µm (n = 12) | 93+/−3 µm (n = 15) | 79.4+/−3.7 µm (n = 11) | 84.0+/−2.4 µm (n = 13) |
| Collateral branches at 30 hpf | 0.08+/−0.08 (n = 7) | 2.1+/−0.2 (n = 10) | 0.12+/−0.08 (n = 17) | 2.7+/−0.4 (n = 14) |
| Collateral branches at 48 hpf | 3.6+/−0.3 (n = 12) | 3.8+/−0.4 (n = 12) | 3.9+/−0.4 (n = 11) | 3.4+/−0.3 (n = 12) |
| Proximal branch position * | 1.0+/−0.05 µm (n = 10) | 0.77+/−0.07 µm (n = 13) | 1.0+/−0.06 µm (n = 17) | 0.53+/−0.04 µm (n = 12) |
| Total protrusions extended 22–30 hpf | 53.5+/−4.5 (n = 12) | 22.4+/−3.1 (n = 10) | 60.9+/−8.3 (n = 12) | 34.1+/−3.0 (n = 12) |
| Rate of forming protrusion 22–30hpf # | 7.4+/−0.7 (n = 12) | 4.2+/−0.5 (n = 10) | 8.6+/−1.2 (n = 12) | 3.8+/−0.16 (n = 12) |
| Ratio of proximal to distal protrusions§ | 0.39+/−0.2 (n = 12) | 0.35+/−0.03 (n = 9) | 0.41+/−0.03 (n = 12) | 0.41+/−0.02 (n = 12) |
| % protrusions that develop into branches 22–38 hpf | 5.0+/−0.5% (n = 12) | 9.7+/−1.4% (n = 9) | 4.6+/−0.8% (n = 12) | 12.9+/−2.1% (n = 12) |
*
Relative Position to wild-type; measured as distance from the cell body to the branch point.
§
Proximal protrusions were scored if they were located between the cell body and the horizontal myoseptum; distal protrusions were scored if they were located between the horizontal myoseptum and the growth cone.
#
Number of protrusions/hour.