Table 1.
Primers used for analysis of genes expression profile
| Enzyme | Acc. no. | Gene name | Primer sequence | Product size (bp) | Annealing temp (°C) |
|---|---|---|---|---|---|
| ADP-glucose pyrophosphorylase small subunit 2b | AK103906 | OsAGPS2b | (F)CTCTGGGTGCCAACTACAGG | 528 | 64 |
| (R)CTTTTGCCCTCACGTCGTC | |||||
| ADP-glucose pyrophosphorylase large subunit 1 | D50317 | OsAGPL1 | (F)ATGCTGGCCCAGACACTCT | 550 | 67 |
| (R)GCTCGACTCTCTCCAACAGG | |||||
| ADP-glucose pyrophosphorylase large subunit 3 | AK069296 | OsAGPL3 | (F)ACCACAGGATGAAGCAAAGG | 498 | 63 |
| (R)TCAGCCAGCAGTTCTCCTCT | |||||
| Starch synthase I | D16202 | OsSSI | (F)GCCATTCCAGATCTCATGC | 500 | 60 |
| (R)TGAAGGCCCATTCGAAGA | |||||
| Starch synthase IIb | AF395537 | OsSSIIb | (F)CTAGAGGCCCCTCCCTTTC | 508 | 65 |
| (R)CCTGCAGCTTCCAGTCGT | |||||
| Starch synthase IIc | AF383878 | OsSSIIc | (F)CAGGGCAGGATATGGAGGTA | 508 | 63 |
| (R)TTGAGCTCCCATGCATAACC | |||||
| Starch synthase IIIa | AY100469 | OsSSIIIa | (F)TCGGAAGAAGGTGGAATCTATG | 618 | 61 |
| (R)TTGTGGCTTTGTCGCAGTATG | |||||
| Starch synthase IIIb | AF432915 | OsSSIIIb | (F)AGCCTCCACTCCGAAACCTA | 516 | 67 |
| (R)CCACTACTTGGCTGGCTTTG | |||||
| Starch synthase IVa | GQ151036 | OsSSIVa | (F)CTTGCAACCAAAGGGAACAT | 550 | 64 |
| (R)GGCTTTTTGCTGCTTTCAAC | |||||
| Starch synthase IVb | GQ151051 | OsSSIVb | (F)CTATGGTTCTGGGACGCAAT | 480 | 63 |
| (R)ACCTAGTCCCTGGCAATGTG | |||||
| Granule-bound starch synthase II | AK067654 | OsGBSSII | (F)CCCTTTCTGTGCGGATTAAC | 492 | 56 |
| (R)CGAGGCATAAAAGGCAGAAC | |||||
| Starch branching enzyme I | EF122471 | OsBE1 | (F)CGCCTGCTGCACAAGAAG | 526 | 64 |
| (R)CCTGATCTGCTGCTGACTGC | |||||
| Starch branching enzyme IIa | AB023498 | OsBEIIa | (F)AAGATGGTGGCTCGAGGAG | 507 | 65 |
| (R)CTAGGTGTTGCCGGTCTGTC | |||||
| Starch branching enzyme IIb | D16201 | OsBEIIb | (F)AACCACGAGTTATCCCACCA | 452 | 64 |
| (R)TTCACCTGGAGCCTGCAC | |||||
| Starch debranching enzyme: Isomerase I | AF142590 | OsISAI | (F)CTTCAACTGTAATCATCCTGTGG | 460 | 60 |
| (R)GATACTGTGCCAAGGTTTCCT | |||||
| Starch debranching enzyme: Isomerase II | AC132483 | OsISAIIa | (F)CACCAGGACCTCTCACAAGG | 553 | 61 |
| (R)GAGAAGCTGGTGGTGTACCG | |||||
| Starch debranching enzyme: Isomerase III | AK101554 | OsISAIIb | (F)AACTGCAACCATCCTGTTGTC | 439 | 61 |
| (R)GGTCGCATAAAGTAAATCCATA | |||||
| Starch phosphorylase H | EF576564 | OsPHOH | (F)GCTGGGCTGAGGAAGGATAG | 483 | 64 |
| (R)CTCGTTGCTGGGTTCTCC | |||||
| Starch phosphorylase L | AF327055 | OsPHOL | (F)GTGTTGTTGGTGGGCATTC | 491 | 62 |
| (R)GCATACCCTTGGAACAAAGC | |||||
| Disproportionating enzyme I | AP004306 | OsDPE1 | (F)ATTCAGGACAGGATGCAAAC | 582 | 62 |
| (R)CATCAGGTGGAACACCACTAA | |||||
| Disproportionating enzyme II | NM_001067082 | OsDPE2 | (F)TGGACAAAATTGGGGTTTTC | 457 | 56 |
| (R)ACGCCGAATACTGTCTTCTTTC | |||||
| Glucan-water dikinase | AK103463 | OsGWD | (F)AGTGGCACCAGAAACTGCAC | 479 | 67 |
| (R)AGGAGAGCCAAGGAGCAAAG | |||||
| Phospho glucan-water dikinase | FN179404 | OsPWD | (F)GTCCCTTCTGGTGCTGTGAT | 525 | 67 |
| (R)GACCTCAGCCTGGACAACC | |||||
| β-amylase | L10345 | OsBAM | (F)CGACGATGCTGGAGAGTACA | 469 | 61 |
| (R)TGCATCATATCGACCGAGTG | |||||
| 18s rRNA | AK105009 | 18s | (F)GTGCAACAAACCCCGACT | 406 | 62 |
| (R)GCTGCTGGCACCAGACTT |