Table 5.
Effect of different levels of casein acid hydrolysate on somatic embryogenesis
| Casein acid hydrolysate (%) | No. of total somatic embryos | No. of globular somatic embryos | No. of mature (cotytledonary) somatic embryos | Fresh wt. (g) of somatic embryos | No. of embryos germinated/20 mature embryos | Germination percentage (%) |
|---|---|---|---|---|---|---|
| Controla | 79.00 ± 5.09b | 22.00 ± 1.87 | 38.00 ± 2.34 | 0.32 ± 0.02 | – | – |
| 1 | 35.00 ± 2.83 | 21.00 ± 2.12 | 4.00 ± 0.70 | 0.14 ± 0.02 | – | – |
| 2 | 44.00 ± 3.93 | 26.00 ± 2.34 | 7.00 ± 0.80 | 0.18 ± 0.03 | – | – |
| 3 | 20.00 ± 2.0 | 15.00 ± 1.58 | – | 0.08 ± 0.01 | – | – |
| 4 | 15.00 ± 2.0 | 12.00 ± 1.22 | – | 0.06 ± 0.02 | – | – |
aLiquid embryogenic medium supplemented with sucrose and PEG (3 % each)
bAverage value ± Standard Error (SE)
– No response