Table 6.
Effect of different inoculum densities on maturation and germination of somatic embryos in C. borivilianum
| Inoculum density (g of cell aggregates/40 ml of liquid medium) | No. of total somatic embryos | No. of globular somatic embryos | No. of mature (cotytledonary) somatic embryos | Fresh wt. (g) of somatic embryos | No. of embryos germinated/20 mature embryos | Germination percentage (%) |
|---|---|---|---|---|---|---|
| 0.10 | 40.00 ± 3.39b | 14.00 ± 1.58 | 16.00 ± 1.22 | 0.16 ± 0.02 | 6.00 ± 0.94 | 30.00 |
| 0.15 (control) a | 66.00 ± 3.87 | 20.00 ± 2.23 | 32.00 ± 2.34 | 0.28 ± 0.03 | 10.60 ± 1.34 | 53.00 |
| 0.20 | 61.00 ± 3.80 | 17.00 ± 1.87 | 28.00 ± 2.00 | 0.24 ± 0.02 | 10.00 ± 1.24 | 50.00 |
| 0.40 | 79.00 ± 5.09 | 22.00 ± 1.87 | 38.00 ± 2.34 | 0.32 ± 0.02 | 11.50 ± 1.22 | 57.50 |
| 0.60 | 55.00 ± 3.80 | 23.00 ± 1.87 | 18.00 ± 1.58 | 0.21 ± 0.02 | 7.00 ± 0.89 | 35.00 |
| 0.80 | 38.00 ± 3.16 | 19.00 ± 1.58 | 8.00 ± 1.00 | 0.15 ± 0.01 | 2.00 ± 0.63 | 12.00 |
| 1.00 | 30.00 ± 2.24 | 21.00 ± 2.12 | 4.00 ± 1.00 | 0.13 ± 0.01 | – | – |
aLiquid embryogenic medium supplemented with sucrose and PEG (3 % each) and inoculum density of 0.15 g/40 ml of medium bAverage value ± Standard Error (SE)
– No response