Table 1.
Comparison between upper, middle and lower segments of semihardwood orthotropic stems of Araucaria excelsa R. Br. var. glauca in response to 2iP in the culture medium after 55 days. MNS/E represent mean number of shoots per explant and MLS/E represent mean length of shoots per explants
| Treatment | MNS/E (SD)a | MLS/E (mm) (SD) | Abnormal shoots* (SD) | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 2iP (μM) | U | M | L | U | M | L | U | M | L |
| 0.00 | 2.33 cd† (0.33) | 0.66 e(0.07) | 0.00 e(0.00) | 8.33 ab(0.03) | 3.66 d–f(0.07) | 0.00 h(0.00) | 0.00 f(0.00) | 0.00 f(0.00) | 0.00 f(0.00) |
| 0.10 | 3.00 b–d(0.00) | 2.66 cd(0.14) | 2.33 cd(0.33) | 7.00 bc(1.00) | 3.41 d–g(0.39) | 1.70 e–h(0.70) | 0.00 f(0.00) | 0.00 f(0.00) | 0.00 f(0.00) |
| 0.20 | 3.33 bc(0.02) | 2.33 cd(0.33) | 0.00 e(0.00) | 9.00 ab(1.00) | 8.33 ab(0.03) | 0.00 h(0.00) | 0.00 f(0.00) | 0.00 f(0.00) | 0.00 f(0.00) |
| 0.30 | 3.66 bc(1.94) | 2.00 cd(1.00) | 0.00 e(0.00) | 6.50 bc(0.05) | 4.83 cd(0.03) | 0.00 h(0.00) | 0.00 f(0.00) | 0.00 f(0.00) | 0.00 f(0.00) |
| 0.40 | 2.66 cd(0.14) | 0.66 c(0.07) | 0.00 e(0.00) | 8.00 ab(1.00) | 3.33 d–g(0.33) | 0.00 h(0.00) | 0.00 f(0.00) | 0.00 f(0.00) | 0.00 f(0.00) |
| 0.50 | 3.33 bc(0.34) | 4.66 ab(0.08) | 2.33 cd(1.27) | 6.75 bc(3.90) | 9.83 a(1.00) | 2.60 d–h(0.50) | 0.00 f(0.00) | 0.00 f(0.00) | 0.00 f(0.00) |
| 0.75 | 3.00 b–d(1.00) | 3.00 b–d(1.00) | 0.33 e(0.03) | 6.60 bc(3.03) | 0.90 gh(0.15) | 0.00 h(0.00) | 0.00 f(0.00) | 2.66 b(0.55) | 1.33 e(0.00) |
| 1.00 | 6.00 a(1.00) | 2.66 cd(0.14) | 1.33 de(0.33) | 1.50 f–h(0.10) | 4.33 c–e(0.33) | 0.03 h(0.01) | 2.33c(0.03) | 1.33 e(0.00) | 1.33 e(0.00) |
| 1.25 | 4.00 bc(1.00) | 3.66 bc(0.34) | 0.66 e(0.07) | 2.83 d–h(1.06) | 1.90 e–h(1.00) | 0.33 h(0.00) | 2.33 c(0.00) | 4.33 a(0.33) | 1.66 d(0.00) |
†In each parameter, means followed by the same letters are not significantly different using Tukey's test at P ≤ 0.05. (U = Upper, M = Middle and L = Lower segments of semihardwood orthotropic stems). *Ranking from 1 (lowest abnormality) to 3 (highest abnormality). a Standard Deviation (SD)